| CTRP6

The C1q/tumor necrosis factor-related proteins (CTRPs) are a highly conserved family and have been found to be composed of 15 members (including CTRP1–CTRP15). C1qTNF6 [C1qTNF-related protein 6, also designated as CTRP6 (C1qTNF-related protein-6)] is a glycoprotein composed of 259 amino acids and has a unique molecular structure, consisting of four domains (signal peptide, short N-terminal variable region, collagen domain and C-terminal C1q domain). It expresses mainly in adipose tissue, lung, stomach and so on. Growing body of evidences suggest that C1QTNF6 regulates cardiac fibrosis, inflammatory reaction, endothelial cell function, fibrogenesis, fatty acid metabolism and carcinogenesis and so on.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human CTRP6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human CTRP6 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108